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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Nickel pyrithione induces apoptosis in chronic myeloid leukemia cells resistant to imatinib via both Bcr/Abl-dependent and Bcr/Abl-independent mechanisms

Fig. 2

NiPT-induced apoptosis is associated with caspase activation. a NiPT leads to caspase-dependent cell apoptosis. KBM5, KBM5R, K562, BaF3-p210-WT, and BaF3-p210-T315I cells were exposed to NiPT at the indicated dose for the indicated duration; cell lysates were then analyzed with western blots for PARP, caspase-3, cleaved-caspase-3, caspase-8, and caspase-9. b, c NiPT reduces mitochondrial membrane potential in CML cells. Cells were treated with different doses of NiPT for 24 h, mitochondrial membrane potential was assessed by rhodamine-123 staining coupled with either fluorescence microscope (b) or flow cytometry (c). Mean ± SD (n = 3).*P < 0.05,**P < 0.01, ***P < 0.001, versus control group. d NiPT induces the release of cytochrome C and AIF in CML cells. KBM5 and KBM5R cells were treated with 0.5 μM NiPT for 1, 3, and 6 h, cytoplasmic proteins were analyzed by immunoblotting with cytochrome C and AIF antibodies. e NiPT downregulates anti-apoptotic proteins in CML cells. KBM5 and KBM5R cells were treated with the indicted doses of NiPT for the indicated duration; cell lysates were then immunoblotted to assess the changes of Mcl-1, XIAP, Bcl-2, Bcl-xl, and survivin

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