Fig. 5

Comparison of 616452 against other TGF-β inhibitors. Various other TGF-β (a) were tested against 616452. Flow cytometry of CB CD34⁺ after 4 day induction (b) revealed inhibitors 616454 and SB431542 had little to no effect and may even negatively impact the differentiation into CD41⁺ cells while 616452 enriches the development by ~25%. The three TGF-b inhibitors induced at the following concentrations: 616454 (4) – 10uM, SB431542(5) - 10uM, 616452(6) – 10uM. When all three inhibitors combined, there is nearly a 100% increase in the enrichment. A ploidy analysis of the cells was run after 4 of induction the chemicals (c) to reveal an improved rate of maturation in terms of ploidy development on both days