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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Identification of a potent small molecule capable of regulating polyploidization, megakaryocyte maturation, and platelet production

Fig. 3

616452 on selectively isolated CD41+ cells CB CD34+ cells were cultured with TPO, SCF, IL-3 for 7 days prior for CD41 selection by flow cytometry (a). Control (DMSO induced) and chemical induced cells were grown(n=2) in StemSpan containing only TPO for 8 days prior to cell cycle analysis (b). In control cells we observed only 6% of the cells increased their ploidy numbers with no cells reaching a ploidy of 16N or greater, while 54% of the chemically induced cells had a nuclei of 4N or greater with a 4.5 fold increase in the number of 4N, 30 fold increase in 8N and the appearance of cells with even greater ploidy reaching as high as 64N within 8 days of culture (c, d). CB CD41+ cells were isolated using flow cytometry and cultured in the absence (e) or presence (f) of 616452(10uM). Cells appeared mostly as single cells on prior to day 6 (e, f) but began to form clusters thereon, while the control had little to none (g). By 8 days of induction, the clusters were large and covered the entire plate (h, i, j). A CD41 staining of these clusters illustrates the composition of these clusters as mainly large MK-like cells(k, l)

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