Fig. 2

Dose and time dependence of 616452. Ploidy analysis of the MK’s derived from CB CD34⁺ cells after 8 and 12 days of culture (a, b). Cells were induced with 616452 (10 uM), and analysis were done on the respective days using PI staining and flow cytometry (n > 5). Dose-dependent analysis (n = 3) was done on CB CD34⁺ cells (c) with 1× concentration as 10 uM. Time-dependence studies (n = 3) used CB CD41⁺ cells that were isolated by flow cytometry (d). Cells were cultured for 6 days and chemicals were removed at certain time points and replaced with media containing only TPO. The control cells were grown entirely with TPO