Fig. 7

LRRC4 inhibits ERK-mediated activation of the downstream substrates to inhibit U251 cell proliferation via the D domain. a U251 cells were transfected with vector, LRRC4 or LRRC4-ΔD plasmid. Western blot showing the phosphorylation level of the ERK downstream substrates ELK1, FOXO3a, and CDC25a. b U251 cells were transfected with vector, LRRC4, or LRRC4-ΔD plasmids and were then subjected to CCK8 assays for the indicated times. ^*LRRC4 vs vector, ^#LRRC4-ΔD vs vector. ^*** p < 0.001; ^## p < 0.01; ^### p < 0.001. c U251 cells were transfected with vector, LRRC4, or LRRC4-ΔD plasmid. Matrigel chamber invasion assay showing the invasion of U251 cells. The data represent the mean±SD of three replicates. Bar in the graph represents the s.e.m. One-way ANOVA, ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001; ^## p < 0.01; ^### p < 0.001