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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: The D Domain of LRRC4 anchors ERK1/2 in the cytoplasm and competitively inhibits MEK/ERK activation in glioma cells

Fig. 6

LRRC4 prevents MEK1/2 binding to ERK1/2 in U251 cells. a Co-immunoprecipitation of MEK1/2 and endogenous ERK1/2 in U251 cells. b U251 cells were transfected with different concentration of GFP-LRRC4. Western blot analysis showed that LRRC4 inhibits ERK1/2 activation, and the inhibition is dose-dependent. c U251 cells were transfected with GFP or GFP-LRRC4. The interaction of MEK 1/2 with ERK 1/2 in the presence of LRRC4 (different titrates) was analyzed by co-immunoprecipitation. d Co-immunoprecipitation of the purified MEK1/2 and ERK1/2 in vitro. The interaction of MEK1/2 and ERK1/2 became stronger with increasing MEK1/2 concentration. e The purified LRRC4 protein prevents MEK binding to ERK1/2 in vitro. The combination of the MEK1/2 and ERK1/2 was reduced with the increase in LRRC4 concentration

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