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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: The D Domain of LRRC4 anchors ERK1/2 in the cytoplasm and competitively inhibits MEK/ERK activation in glioma cells

Fig. 4

LRRC4 inhibits ERK1/2 activation. a HEK293 cells were transfected with GFP or GFP-LRRC4 followed by EGF (50 ng/mL) stimuli. Western blot analysis showed that LRRC4 inhibited the phosphorylation of ERK1/2 with EGF stimuli. b HEK293 cells were transfected with GFP or GFP-LRRC4 followed by PMA (1 μM) stimuli. Western blot analysis showed that PMA has no effect on the activation of ERK1/2 with or without LRRC4 in the HEK293 cells. c HEK293 cells expressing GFP were treated with EGF (50 ng/mL). Western blot analysis showed that EGF stimulation promoted increased expression of phosphorylated ERK1/2 in both the cytoplasm and nucleus with EGF stimuli. d HEK293 cells were transfected with GFP or GFP-LRRC4 followed by EGF (50 ng/mL) stimuli. Western blot analysis showed that LRRC4 inhibited the phosphorylation of ERK1/2 in both the cytoplasm and nucleus with EGF stimuli. e HEK293 cells were transfected with GFP or GFP-LRRC4 without EGF (50 ng/mL) stimulation. Western blot analysis showed that LRRC4 had no effect on pERK1/2 expression in both the cytoplasm and nucleus without EGF stimuli. The data represent the mean±SD of three replicates. Bar in the graph represents the s.e.m. Student’s t test, NS : no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001

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