Fig. 2

CD274 promotes cell cycle entry of LICs. a Representative flow cytometric plot for the percentages of Mac-1⁺/c-Kit⁺ cells (enriched for LICs) in the recipient mice upon primary transplantation. b Quantification results for panel a (n = 3). c Representative flow cytometric plot for the percentages of Mac-1⁺/c-Kit⁺ cells in the recipient mice upon secondary transplantation. d Quantification results for c (n = 3). e–g Representative images for the colony forming units of WT and CD274-null AML cells upon secondary transplantation. Colony numbers (f) and total cell number of colonies (g) in e were evaluated (n = 3, right). h, i Representative flow cytometric plots for cell cycle in WT and CD274-null Mac-1⁺/c-Kit⁺ cells (enriched for LICs) determined by Pyronin Y and Hoechst 33342 staining upon secondary transplantation (h). Quantitative data for panel h (i, n = 3)