Fig. 3
MYC promotes the SRR2 probe binding and the transcriptional activity of Sox2. a SRR2 probe pull-down assay was performed in RU and RR cells originated from Karpas 299 cells to compare the bindings between Sox2, MYC, and SRR2 probes. The western blots in the right panel showed the input of the pull-down assay. b The SRR2 probe pull-down assay was performed to assess the Sox2, MYC, and SRR2 binding in RU cells with MYC transient transfection, as compared to cells with EV transfection. The western blots in the right panel showed the input of the pull-down assay. c The relative mRNA levels of Sox2 downstream target genes such as WNT2B, CTNNB1, and BCL9 in RU cells originated from SupM2 with EV or MYC transient transfection at 48 h. d RR cells derived from SupM2 were subjected with 10 μM MYC inhibitor 10074-G5 for 0, 4, 6, 8, and 24 h, and then the SRR2 probe pull-down assay was performed. The western blots in the right panel showed the input. e RR cells originated from both cell lines were subjected with 10 μM 10074-G5 for 8 h, then qRT-PCR assay was performed to assess the mRNA levels of MYC, CTNNB1, and BCL9. f The SRR2 luciferase activity in RU and RR cells originated from SupM2 with EV or pcDNA-SOX2-FLAG (i.e., SOX2-FLAG) transfection at 48 h. The western blots showed the transfection efficiency of SOX2-FLAG. g The Sox2-SRR2 binding ability in RU and RR cells originated from SupM2 with EV or SOX2-FLAG transfection at 48 h