Fig. 2
Metabolic profile of the AML mouse-human model. a Design of the study. b Leukemic cell count (human CD33+, CD45+ values) over time. c OPLS-DA score plot for healthy mice (tp1M and tp2M; green circles; n = 12) vs mice at tp5M and tp6M (blue circles; n = 12) showing an AML metabolic signature at an early stage of AML. OPLS-DA model with N = 24, CV-ANOVA p = 0.002, R 2 = 0.99, and Q 2 = 0.819. The area under the curve (AUC) of the ROC analysis was 0.96 (p < 0.001). d Metabolites discriminating healthy and human AML-engrafted mice. Positive loading values (blue bars) indicate the metabolites increased in AML mice, while negative values (green bars) are associated with increased levels in healthy mice