Fig. 3
From: DNMT3A mutation leads to leukemic extramedullary infiltration mediated by TWIST1
CNS-infiltrating leukemic cells concentrate in murine meninges. a HE staining of brain biopsies taken from control or transplanted mice. Meningeal spaces are boxed in black. Myeloid cells infiltrate into brain meninges of OCI-AML3 group. b HE staining of spinal cord biopsies from control or transplanted mice. Enlarged black boxes represent spinal meninges. Xenografts distributed along the spinal meninges are detected in transplanted mice. c Immunohistochemical staining of murine brain and spinal cord. hCD44+ cells in brown, pointed by red arrows, indicate the OCI-AML3 cells in transplanted mice. Black triangles and squares denote cerebral parenchymas and spinal cavities, respectively. Dashed lines indicate the meninges. d Immunofluorescence shows murine brain meninges infiltrated by OCI-AML3 labeled by GFP antibody