Table 2 Mismatches against consensus germline reverse primers and/or probes
Cases | Mismatches against primers | Mismatches against probes | ||||
|---|---|---|---|---|---|---|
Primers | Primer binding site sequences | No. | Probes | Probe binding site sequences | No. | |
Dx53 | R-JH6-intron | gcagaGaGGaaGggccctagagt | 4 | T-JH6 | cacggtcaccgtctcctcaggCaagaa | 1 |
M3 | R-JH4-intron | cagagAAaaTATCACagagaggttgt | 8 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M8 | R-JH4-intron | cagagCtaaagcaggagagaggttgt | 1 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M4 | R-JH4-intron | cagGAttTaagTaggGgagaCgttgt | 6 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
Dx31 | R-JH4-intron | TagaCCCaaagTaggagagaCAttAt | 8 | T-JH1.2.4.5 | Tcctggtcaccgtctcctcaggtg | 1 |
M5 | R-JH3-intron | aggcagaaggaaagccatcttac | 0 | T-JH3 | cCagggacaatggtcaccgtctcttca | 1 |
M10 | R-JH6-intron | gcagaaaacaaaggccctagagt | 0 | T-JH6 | cacggtcaccgtctcctcaggtaagaa | 0 |
Dx49 | R-JH6-intron | – | ? | T-JH6 | cacggtcaccgtctcctcaggtaagaT | 1 |
M1 | R-JH4-intron | – | ? | T-JH1.2.4.5 | cTGt--- | ≥2 |
M6 | R-JH3-intron | – | ? | T-JH3 | caagggacaat--- | ? |
M2 | R-JH4-intron | cagagttaaagcaggagagaggttgt | 0 | T-JH1.2.4.5 | ccctggtcGccgtctcctcaggtg | 1 |
M7 | R-JH5-intron | agagagggggtggtgaggact | 0 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M9 | R-Jk1 | gatcacttcatagacacagggaacag | 0 | T-Jk1 | tggaaatcaaacgtgag | 0 |