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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: The novel thiosemicarbazone, di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), inhibits neuroblastoma growth in vitro and in vivo via multiple mechanisms

Fig. 3

The effects of Dp44mT, DpC, and L1 on apoptosis in four neuroblastoma cell lines, namely SK-N-LP, BE(2)C, SK-N-AS, and SH-SY5Y, as judged by flow cytometric analysis. Cells were incubated with either control media alone (Con), control media containing DMSO (DMSO), DpC, Dp44mT, or L1 (25 μM) for 24 h/37 °C. Apoptosis was examined using Annexin V/PI staining by flow cytometry. Cells that were Annexin V+/PI+ (top right quadrant) were defined as being in late apoptosis, while cells being Annexin V+/PI– (bottom right quadrant) were considered to be in early apoptosis. Cells that were Annexin V–/PI+ (top left quadrant) were considered to be necrotic and those that were negative for both (bottom left quadrant) were viable cells. The values shown represent the percentage of cells in each quadrant. Results show a typical experiment of three performed

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