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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Interleukin-15 enhances the proliferation, stimulatory phenotype, and antitumor effector functions of human gamma delta T cells

Fig. 2

Distinct Th1 cytokine release and marked tumor cell killing by IL-15+IPP activated γδ T cells. a γδ T cell invigorated for 5 days with IL-15 and IPP (dark bar) or IL-2 and IPP (gray bar) were stimulated for 4 h with U266 cells (E:T = 5:1). Bar graphs represent the cytokine secretion level of 1 million γδ T cells (pg/mL), as determined by ECLIA (IFN-γ, TNF-α, IL-5, IL-10, and IL-17) and ELISA (TGF-β). Data are depicted as mean of duplicate measurements of six independent donors. Wilcoxon matched-pairs signed rank test. b γδ T cells stimulated for 5 days with IL-15+IPP (filled squares) or IL-2+IPP (filled circles) were analyzed by flow cytometry for cytotoxicity against the tumor cell lines Daudi and U266. Target cell killing was determined by annexin V/PI staining after 4-h incubation at different E:T ratios. Results are expressed as mean percentage killing, calculated using the formula specified in the “Methods” section. Data are from six different experiments involving 6–12 different donors. Paired t test. **p < 0.01; *p < 0.05

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