Fig. 5

miR-34a negatively regulates stem cell-like properties of gastric cancer cells. a Expression of miR-34a was decreased in microsphere of MKN45 cells. Fold change of miR-34a in spheroid cells (SC) and parental cells (PC) was analyzed by QRT-PCR. b miR-34a overexpression inhibits cell growth in MNK45 cells. Cells growth was detected by CCK-8 in miR-34a overexpressing cells (miR-34a) and control cells (Con). c miR-34a overexpression inhibits proliferation in MNK45 cells. Proliferation ability of cells was detected by colony formation assay, then photographed (upper panel) and quantified (lower panel). d miR-34a overexpression decreases microsphere formation in MKN45 cells. Microsphere formation was tested by serum-free suspension culture (upper panel) and quantified (lower panel) in miR-34a overexpressing cells (miR-34a) and control cells (Con). e miR-34a overexpression inhibits migration ability in MNK45 cells. Migration ability of cells was detected by Transwell Assay and then photographed (upper panel) and quantified (lower panel). f miR-34a overexpression decreases drug resistance to EPI in MKN45 cells. Cell viability after treatment with increasing doses of drug for 48 h was determined with CCK-8. g miR-34a overexpression in MKN45 cells after infecting by lentivirus containing miR-34a was confirmed by QRT-PCR. Error bars in all panels represent the mean ± SD (*P < 0.05, **P < 0.01 as compared with control)