Fig. 2

Bmi-1 regulates the expression of several miRNAs. a Bmi-1 overexpression upregulates the expression of miR-21, miR-34a, and miR-125a-5p. Fold change of miRNAs was detected by QRT-PCR in SGC7901 cells with Bmi-1 overexpression and control cells. b Bmi-1 knockdown downregulates the expression of miR-21, miR-34a, and miR-125a-5p. Fold change of miRNAs was detected by QRT-PCR, normalized to 5S in MKN45 cells with Bmi-1 knockdown and control cells. c Pearson correlation analysis showed there was a positive correlation between the expression level of Bmi-1 and miR-21. The expression of miR-21 in gastric cancer tissues with high Bmi-1 expression was higher than tissues with low Bmi-1 expression. The log10 of miR-21 expression level was plotted in Bmi-1 high and Bmi-1 low groups of gastric cancer tissues. d The expression of miR-34a was not different in gastric cancer tissues with high Bmi-1 expression from tissues with low Bmi-1 expression. The log10 of miR-34a expression level was plotted in Bmi-1 high and Bmi-1 low groups of gastric cancer tissues. Error bars in all panels represent the mean ± SD (*P < 0.05, **P < 0.01)