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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models

Fig. 2

TIP30 was regulated by HIF-2α at protein level in HCC cell lines. Lentivirus infection was used to establish a stable knocking-down or overexpression of HIF-2α in MHCC97H cell lines. The relationship between HIF-2α and TIP30 was detected by Western blot. a Knocking-down of HIF-2α upregulated TIP30 expression. b Knocking-down of TIP30 did not influence the expression of HIF-2α. c, d Metformin in combination with sorafenib synergistically inhibited HIF-2α protein expression and subsequently upregulated TIP30 protein expression. e MHCC97H cells were exposed to CoCl2 (400 μM) for 6 h, and anti-HIF-2α or anti-IgG was used for immunoprecipitation. Immunoprecipitated and purified DNA together with 1 % of input DNA were used for PCR amplification of a 214-bp product encompassing HRE region of TIP30 promoter

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