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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets

Fig. 3

H2S-induced promotion of megakaryocytes/platelets generation and improvement of survival are blunted in c-mpl−/− mice. a Without radiation exposure, NaHS treatment (20 μmol kg−1 day−1) for 14 days caused an increase in circulating platelet levels in WT mice but not in c-mpl−/− mice. b Flow cytometry showed that NaHS treatment (100 μmol L−1) failed to increase the number of megakaryocytes identified as CD61-positive cells in cultured fetal liver cells isolated from c-mpl−/− mice. c Circulating platelets were significantly decreased in both WT and c-mpl−/− mice exposed to radiation at day 7. NaHS treatment (100 μmol kg−1 day−1) increased platelet levels in WT mice but not in c-mpl−/− mice. d Morphological examination of the bone marrow showed that NaHS treatment (100 μmol kg−1 day−1) failed to increase the number of megakaryocytes (black marrows) in c-mpl−/− mice. Scale bar = 250 μm. e Bleeding was not ameliorated with NaHS treatment (100 μmol kg−1 day−1) in c-mpl−/− mice exposed to radiation at day 7. f Likewise, fecal occult blood was not ameliorated with NaHS treatment (100 μmol kg−1 day−1) in c-mpl−/− mice exposed to radiation at day 7. g NaHS treatment (100 μmol kg−1 day−1) failed to improve survival in c-mpl−/− mice exposed to radiation as compared to vehicle-treated mice. *P < 0.05 versus vehicle-treated mice exposed to radiation, log-rank test. Data in the graphs are means ± SEM. P values less than 0.05 represent statistical significance

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