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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets

Fig. 2

H2S treatment promotes generation of megakaryocytes/platelets in cultured fetal liver cells. a NaHS treatment caused a concentration-dependent increase in megakaryocytes which were identified as CD61-positive cells using flow cytometry in fetal liver cells isolated from WT mice. b Platelets were identified as CD41-positive and 7-AAD-negative signals using flow cytometry. Platelet counts were significantly increased with NaHS treatment at 50 and 100 μmol L−1 in the culture medium of fetal liver cells. TPO treatment (10 ng mL−1) also increased platelet counts. c Morphology of megakaryocytes in cultured fetal liver cells were shown with a confocal fluorescence microscopy with staining of DAPI, CD41-FITC, and CD61-PE. Scale bar = 50 μm. d Scanning electron microscopy revealed pseudopod formation (blue arrow) and membrane blebbing (yellow arrow) in the megakaryocytes identified in cultured fetal liver cells treated with NaHS (100 μmol L−1). Data in the graphs are means ± SEM. P values less than 0.05 represent statistical significance

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