Fig. 5
From: Haplo-insufficiency of both BubR1 and SGO1 accelerates cellular senescence
a BubR1 +/− SGO1 +/− MEFs express enhanced levels of p21. MEFs of various genetic backgrounds (wildtype, BubR1 +/−, SGO1 +/−, and BubR1 +/− SGO1 +/−) were cultured and passed in vitro as described in the Methods section. MEFs of different passages were collected and lysed. Equal amounts of cell lysates were blotted for p21 and β-actin. b The same set of lysates were blotted with antibody to p16