Table 4 Top regulated pathways in the list of upregulated genes in AML post MDS mice

Cell metabolism

Oxidative phosphorylation

18

1.0E-03

NDUFB5, NDUFB6, NDUFB8, ATP5B, COX4I1, NDUFC1, COX5A, LHPP, NDUFA10, ATP5G3, PPA2, NDUFB2, ATP6V0C, ATP6V1A, UQCR11, NDUFV1, SDHC, COX6B2

NDUF family: NADH dehydrogenase (ubiquinone) Fe-S family

SDHC: succinate dehydrogenase complex, subunit C

LHPP: phospholysine phosphohistidine inorganic pyrophosphate phosphatase

Nucleotide metabolism Pyrimidine metabolism

14

2.9E-03

POLR2G, NUDT2, DTYMK, POLE, AK3, POLA1, POLR2C, NME4, TYMP, POLE3, NME1, POLD1, ENTPD1, DUT

NUDT2: nucleoside diphosphate, AK3: adenylate kinase

TYMP: thymidine phosphorylase, NME: nucleoside diphosphate kinase

POLE, POLR2C, POLE3, PLD1: polymerases, DUT: deoxyuridine triphosphate

Immune system

Toll-like receptor signaling

14

3.8E-03

MAP2K3, MAP2K4, TLR4, FADD, CXCL11, TLR7, CCL4, TLR8, CD80, IFNA7, MAPK13, IFNA5, TICAM2, PIK3R2

MAP2K3, MAP2K4: MEK proteins, TICAM2: TIR domain-containing adapter molecule 2

PIK3R2: Phosphatidylinositol 3-kinase regulatory subunit beta

Cellular processes

Cell cycle

16

5.8E-03

CDC6, E2F3, CCNH, SKP2, YWHAB, ESPL1, CDC20, CHEK1, CHEK2, CDC25C, MCM4, 2CDK2, CCNE2, CDC45, MAD2L1, CDKN2C

CCNH: cyclin H; SKP2: S-phase kinase-associated protein 2; YWHAB: 14-3-3 protein beta/alpha

ESPL1:separin, CCNE2: cyclin E2, MAD2L1: MAD2 mitotic arrest deficient-like 1

CDKN2: cyclin-dependent kinase inhibitor 2A (p16)

Signaling molecules Cytokine-cytokine receptor

25

5.9E-03

IL1R1, ACVRL1, PDGFB, IL13, CXCL11, CCL4, TNFRSF4, IL10, IL12RB2, CCL22, IFNA7, IFNA5, TNFRSF18, IFNGR1, EPO, IL4, IL23R, IL21, CCL17, TNFRSF9, INHBA, CCR8, TNFSF11, CXCL13, CCR2

IL1R1, IL12RB, I L23R,IL21: interleukines/interleukine receptor

CCL4, CCL22, CCL17, CXCL11, CXCL13 CCR2, CCR8: chemokine

TNFRSF4, TNFRSF18, TNFRSF9, TNFSF11. Tumor necrosis receptor

IFNA7, IFNA5, INFGR1, INHBA: interferon signaling

EPO: erythropoietin

Signal transduction

JAK-STAT signaling pathway

15

5.0E-02

IL4, IL23R, SOCS2, IL13, IL21, IL10, IL12RB2, SPRY1, IFNA7, IFNA5, SPRED2, IFNGR1, IL13RA2, EPO, PIK3R2

IL4, IL23R, IL13, IL21, IL10, IL12RB2, IL13RA2: interleukines/interleukine receptor

IFNA7, IFNA5, IFNGR1: interferon signaling

EPO: erythropoietin

PIK3R2: phosphoinositide-3-kinase, regulatory subunit 2

Genetic information processing. Replication and repair

5

6.4E-02

BLM, RAD51L1, POLD1, RAD54B, RAD51

BLM: bloom syndrome protein, POLD1: polymerase delta

RAD51L1, RAD54B, RAD51: recombinases family

Genetic information processing

11

6.8E-02

HIST1H2AC, C4A, H2AFV, CD80, HIST2H2BE, HIST2H2AC, HIST1H2BH, HIST1H2AI, SNRPB, H2AFY, FCGR1, IL10

HIST1H2AC,H2AFV,HIST2H2BE,HIST2H2AC,HIST1H2BH, HIST1H2AI, H2AFY: histone proteins

C4A: complement factor 4; CD80, costimulatory factor, SNRPB: small nuclear ribonucleoprotein-associated proteins B, FCGR1: IgG Fc receptor