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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Fig. 6

a SEM imaging of the explanted scaffolds 4 weeks after transplantation. White arrows: erythrocytes; black arrows: T cells; red arrows: thrombocytes; gray arrow: neutrophils/granulocytes; asterisk: β-TCP. b-c Gating strategy for the flow cytometry: Hematopoietic stem (LSK) and progenitor cells (LK) were identified by being negative for lineage markers and by the expression of Sca1 and c-kit. Lineages were identified by the following stainings: granulocytes (Gr1+CD11b+), monocytes (Gr1CD11b), erythroid precursor cells (Gr1CD11bCD3CD19), T cells (Gr1CD11bCD3+), B cells (Gr1CD11bCD19+). d Flow cytometry analysis of the explanted scaffolds 4 weeks after transplantations. Surface marker expression is shown as a percentage of the viable cell population; mean ± SD of three independent experiments

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