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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Fig. 3

Hematopoietic stem cell characterization in β-TCP scaffolds in vitro. C-kit+ cells were subjected to the different β-TCP/matrix hybrids over 14 days in vitro. a Percentage of DAPI-viable cells after 14 days of c-kit+ cell culture with and without collagen I/III/Matrigel® on β-TCP scaffolds in the presence of MSC support. b Expansion/decrease of linSca1+c-kit stromal cells normalized to day 4 of the culture. c Expansion/decrease of lin normalized to day 4 of the culture. Mean ± SD of three independent experiments. d Colony-forming potential of cells isolated from 500- and 800-μm β-TCP scaffolds with and without collagen I/III/Matrigel® in the presence of MSC support at day 4 and day 14. Results are mean ± SD of three independent experiments. CFU colony-forming unit, GEMM granulocyte-erythrocyte-macrophage-megakaryocyte, GM granulocyte-monocyte. without

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