Fig. 5

G1XP lymphomas harbor segmental translocations involving Pvt1 locus. a Metaphase FISH analysis of the Igh-Pvt1 translocation in G1XP lymphoma (119 J). Top: Diagrams of Igh or Pvt1 FISH probes. Bottom left: five copies of the 5′ (green) and 3′ (red) Pvt1 probes are co-localized (yellow arrows); Bottom middle: three copies of the 3′Igh (green) and 3′Pvt1 probes are co-localized (white arrows); Bottom right: five copies of 5′Pvt1 probe (green) and two copies of 5′ Igh probe (red) are not co-localized (indicated by green or red arrows, respectively). b Quantification of Igh-Pvt1 translocations analysis in G1XP lymphoma (119 J). The number of analyzed metaphases is indicated. c Schematic map of t(15;12;15) translocation in G1XP 119 J. Chr15 is in red and the Pvt1 locus is depicted in blue line with the 5′ and 3′ flanking FISH probes indicated. The inserted region of Chr12 is in green encompassing the region from Sα (junction 2) to Sμ (junction 1) as shown in Fig. 3d. The breakpoints of Pvt1 locus are adjacent to each other in these two CTX junctions with only 143 bp deleted in the Pvt1 locus