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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Combined deletion of Xrcc4 and Trp53 in mouse germinal center B cells leads to novel B cell lymphomas with clonal heterogeneity

Fig. 1

Deletion of Xrcc4 but not Trp53 via Cγ1cre leads to a high level of genomic instability at the Igh locus. a Quantification of Igh abnormalities in day 4 anti-CD40/IL4-activated wt (n = 6), Cγ1-p53c/c (n = 4), Cγ1-X4c/c (n = 3), and DKO (n = 6) splenic B cells. Data are presented as mean ± s.e.m. Statistical analyses were calculated by a Student’s t test with two-tailed distribution and equal variance. p = 0.024 (control vs Cγ1-p53c/c), p = 6.78E-05 (control vs Cγ1-X4c/c), p = 1.03E-06 (control vs DKO), p = 0.805 (Cγ1-X4c/c vs DKO). b Top: Diagram of Igh metaphase FISH probes and abnormalities. An intact Igh shows co-localized red and green signals while a broken locus appears as split red and green signals. Bottom: Example of metaphase FISH showing Igh breaks indicated with white arrows

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