Fig. 6
From: Roles of palmitoylation and the KIKK membrane-targeting motif in leukemogenesis by oncogenic KRAS4A
KIKK motif of KRAS4A functions by providing a positive charged region. a Morphology of cultured NIH3T3 cells stably expressing KRAS4AG12D/C180S with KIKK motif mutated to QIKK, KIQK, KIKQ or QIQK, QIKQ, KIQQ was observed under light microscope (original magnification, ×200). b, d Quantification of anchorage-independent growth of NIH3T3 cells expressing the indicated proteins. Colonies were counted on day 14 after plating in triplicate. Student t test was used for the statistical analysis. ****P < 0.0001. c Morphology of cultured NIH3T3 cells stably expressing KRAS4AG12D, KRAS4AG12D/C180S, KRAS4AG12D/QIQQ, or KRAS4AG12D/C180S/QIQQ was observed under light microscope (original magnification, ×200). e Serum-starved lysates of NIH3T3 cells expressing the indicated proteins were analyzed by western blotting to detect the phosphorylation levels of c-RAF, MEK, ERK1/2, AKT, and S6RP. Expressions of RAS, GFP, and β-actin were used as loading controls