Skip to main content
Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Roles of palmitoylation and the KIKK membrane-targeting motif in leukemogenesis by oncogenic KRAS4A

Fig. 1

Effects of palmitoylation on leukemogenesis of oncogenic NRAS vs. KRAS4A. a The expression levels of KRAS4A in KRAS-mutated leukemia cell lines indicated were detected by western blot analysis using an anti-KRAS4A specific antibody. Total RAS and actin were detected as controls. b Quantification of the relative abundance of KRAS4A and KRAS4B slice variants in bone marrow cells from AML patients with oncogenic KRAS mutations. The results are presented as means ± SD. c Kaplan-Meier plot of cumulative survival of recipient mice transplanted with bone marrow cells infected by KRAS4AG12D, KRAS4AG12D/C180S, NRASG12D, or NRASG12D/C181S containing retroviruses. The observation period was over 1 year after BMT. The median survival is 23, 26, and 86 days for KRAS4AG12D, NRASG12D, and KRAS4AG12D/C180S, respectively. Disease incidences: KRAS4AG12D (9/10 CMML; 1/10 AML), KRAS4AG12D/C180S (2/10 CMML; 8/10 T-ALL), NRASG12D (5/10 CMML; 5/10 AML). Arrows represent the two moribund KRAS4AG12D/C180S mice diagnosed as CMML-like disease. P values were determined by the log-rank test. ****P < 0.0001; n = 10 in each group. d Representative analyses of leukemic bone marrow cells from the moribund KRAS4AG12D/C180S mice with T-ALL like disease (8 out of 10) by flow cytometry are shown. The MGI vector mice were used as control. e Comparison of CD4+, CD8+ cell percentages in GFP+ BM cells from the moribund KRAS4AG12D/C180S mice with T-ALL like disease (n = 4) and the MGI vector mice (n = 4). Student t test was used for the statistical analysis. *P < 0.05. f Representative thymus from moribund KRAS4AG12D/C180S mice with T-ALL like disease and that from the MGI vector mice. g Comparison of weight of thymus from moribund KRAS4AG12D/C180S mice with T-ALL like disease vs. that from MGI vector mice. The data were presented as mean ± SEM; *P < 0.05; n = 3 in each group. h Representative analyses of leukemic bone marrow cells from moribund KRAS4AG12D/C180S mice with CMML-like disease (2 out of 10). The MGI vector mice were used as control

Back to article page