Table 2 Synopsis of data on the JIH-5 cell line
Parameter | JIH-5 |
|---|---|
Clinical data | |
Patient | 21-year-old female |
Diagnosis | MPAL |
Treatment status | At the second relapse |
Specimen | BM |
Year of establishment | 2009 |
Culture characterization | |
Culture medium | IMDM + 20 % FCS |
Growth pattern | Single cells in suspension |
Doubling time | 97 h |
Optimal cell density | 1 × 106cells/ml |
Optimal split | 1:3 every 3–4 days |
Cryopreservation | In 70 % medium, 20 % FCS, 10 % DMSO |
Morphology | medium-sized spheroidal morphologies |
Viral status | Negative for EBV |
Contamination | Negative for mycoplasma |
Authentication | Yes (by DNA finger printing, cytogenetic characteristics, immunoprofile) |
Immunoprofiles | |
Myelocytic | CD13+, CD33+, CD15+, MPO+ |
B lymphoid | CD10+, CD19+, CD22+, CD23+, cCD79a+ |
Megakaryocytic | CD41+ |
Progenitor | CD38+, HLA-DR+ |
Plasma cell | CD138+ |
Genetic characterization | |
Karyotypic analysis in conjunction with SKY | 46,XX,del(2)(q33)t(2;2)(p22;q37), t(4;5)(q35;q35),t(5;8)(q32;q22), der(6)del(6)(p21p22)t(6;10)(p23;q23), t(7;21)(p15;q21,der(9)del(9)p21)del(9)(q34.2), der(10)t(6;10),t(12;22)(p13;q13), der(17)t(17;17)(p13;q22),del(19)(q13) |
Array-CGH | del(2)(q33.1-q37.3), del(6)(p21.2-p21.31), del(8)(q21.2), del(8)(q23.3-q24.11), del(9)(q21.33-q34.12), del(10)(q23.33-q24.1), del(10)(q25.1), del(12)(p13.2), del(19)(q13.32), amp(17)(q21.32-q25.3) |
Next-generation sequencing | |
Whole-exome sequencing | Somatic mutations in ABCA8, BCHE, CALCA, CSTF2, FPR1, KCNJ8, MAFB, STMN1, TAAR8 |
Transcriptome sequencing | EP300-ZNF384, CHD4-EP300, MSH2-NLK, HACL1-COLQ, HDAC8-CITED1, POLA2-CDC42EP2 |