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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy

Fig. 1

Membrane-permeable and reduction-sensitive peptide produced free CRGT peptide upon entering platelet cytosol. a Schematic presentation shows that the reduction of disulfide bonds led to a separation of the FAM fluorescence donor and the TAMRA fluorescence acceptor that dequenched the fluorescence of FAM allowing detection by a spectrofluorometer. b Washed platelets were treated with the reduction-sensitive fluorescent peptide. The fluorescence at 576 nm (TAMRA) and 519 nm (FAM) increased over time. c The ratio of FAM/TAMRA fluorescence quantum was calculated and presented as the mean and SD of three independent experiments. d Comparison of the FAM to TAMRA fluorescence ratio among peptide-treated platelets (peptide + platelets), 0.3 M β-ME-treated peptide (peptide + β-ME), or untreated peptide (peptide). The data were measured and presented as same as in panel c. e Confocal analysis of the peptide-treated platelets were performed utilizing Z-stack scanning with intervals of 1.2 μm (from i to iv). Images are from a single experiment representative of three so performed, scale bar represents 2 μm

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