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Table 4 Correlation of cellular MTX polyglutamate levels with mRNA expression of FPGS and FPGH as well as FPGS activity

From: Methotrexate resistance in relation to treatment outcome in childhood acute lymphoblastic leukemia

 

Precursor B cell ALL

T cell ALL

Total ALL

 

MTX-Glu1–6

MTX-Glu4–6

MTX-Glu1–6

MTX-Glu4–6

MTX-Glu1–6

MTX-Glu4–6

FPGS mRNA

  R

−0.168

−0.082

0.345

0.002

0.264

0.280

  P value

0.456

0.724

0.176

0.994

0.104

0.104

 Number of patients

22

21

17

14

39

35

FPGH mRNA

  R

0.117

0.195

0.366

0.537

0.101

0.045

  P value

0.622

0.424

0.219

0.110

0.576

0.817

 Number of patients

20

19

13

10

33

29

FPGS activity

  R

0.429*

0.419*

0.265

0.05

0.522*

0.533*

  P value

0.005

0.01

0.431

0.898

<0.001

<0.001

 Number of patients

41

37

11

9

52

46

  1. R and Pvalues were estimated using Spearman’s Rho test; the concentration of MTX polyglutamates (MTX-Glu1–6 and MTX-Glu4–6) is expressed as pmol MTX-Glun/109 cells; mRNA expression of folate enzymes is expressed as ratio normalized to β-actin; FPGS activity is expressed as pmol MTX-Glu2 formed/h/106 cells. The asterisk denotes significant (P < 0.05) associations