Fig. 1

Relative ANRIL expression in HCC tissues and HCC cell lines and ANRIL was regulated by SP1. a Relative ANRIL expression in HCC tissues (n = 77) compared with corresponding non-tumor tissues (n = 77). ANRIL expression was examined by qPCR and normalized to GAPDH expression. Results were presented as ΔCT in tumor tissues relative to normal tissues. b ANRIL expression was classified into two groups. Positive ΔΔCT meant high ANRIL expression. Negative ΔΔCT meant low ANRIL expression. c Relative ANRIL expression levels of HCC cell lines (HepG2, Hep3B, MHHC-97H) compared with those of the normal hepatic epithelium cell line (L02). d ChIP-qPCR of SP1 occupancy and binding in the ANRIL promoter in HepG2 and Hep3B cells and IgG as a negative control. e The SP1 expression level was determined by qPCR when HepG2 cells transfected with si-SP1. f The ANRIL expression level was determined by qPCR when HepG2 cells transfected with si-SP1. g The SP1 expression level was determined by qPCR when Hep3B cells transfected with si-SP1. h The ANRIL expression level was determined by qPCR when Hep3B cells transfected with si-SP1. i The SP1 expression level was determined by qPCR when HepG2 cells transfected with EGFP-SP1. j The ANRIL expression level was determined by qPCR when HepG2 cells transfected with EGFP-SP1. k The SP1 expression level was determined by qPCR when Hep3B cells transfected with EGFP-SP1. l The ANRIL expression level was determined by qPCR when Hep3B cells transfected with EGFP-SP1.*P < 0.05, **P < 0.01