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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy

Fig. 7

Angptl4 induces de novo STAT3 expression in CD61+CD41low/negative MKs in vitro. a mRNA expression of the transcription factors Fli-1, STAT3, NF-E2, and GATA-1 in SCF-, SCF + TPO-, SCF + Angptl4-, and SCF + TPO + Angptl4-supplemented cultures at day 5 after initiating cultures from 1 × 105 Lin− BM cells. Mean ± SEM of three different experiments with a total of 12 SCF-; 12 SCF and TPO-; 12 SCF and Angptl4-; and 12 SCF, Angptl4, and TPO-supplemented cultures are shown. Statistically significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001). b, c Representative FACS profile and gating strategy for the detection of STAT3 expression of CD61−CD41−, CD61+CD41low/negative, and CD61+CD41high cells in cytokine-supplemented cultures at day 5 after initiating cultures from 1 × 105 Lin− BM cells. c Upper panel: initiating cells are purified from STAT3-YFP knockin mice; lower panel: initiating cells stem from WT mice. Statistically significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001)

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