Fig. 2

Angptl4 is upregulated in the BM of mice during inflammatory conditions. a Hematoxylin-eosin staining and Angptl4 expression in BM sections from WT and TLR-4^−/− mice at 72 h after double PBS or LPS (50 μg from 1:1 mixture of E. coli strain K12 and S. minnesota strain R595) injections. Sections were either labeled with anti-Angptl4 antibody (right panel) or isotype-matched antibodies (left panel). Original magnification ×200. b G-csf and Angptl4 mRNA expression in the BM of PBS-treated (white bars) or LPS-treated (gray bars) WT mice. Expression levels are normalized against 18 s RNA. Mice were i.p. injected once with 50 μg LPS and analyzed 8 h later. Mean ± SEM of three different experiments each with a total of three PBS- and three LPS-injected mice/group are shown. c Blood and BM plasma G-CSF and Angptl4 protein levels in the PBS-treated (white bars) or LPS-treated (gray bars) WT mice, treated as in a. Mean ± SEM of two different experiments with a total of five PBS-treated and five LPS-treated mice are shown. d G-CSF and Angptl4 protein levels in supernatants at 48 h after single PBS (white bars) or LPS stimulation (10 μg from 1:1 mixture of E. coli strain K12 and S. minnesota strain R595, gray bars) of BMSC cultures. Mean ± SEM of supernatants from three experiments, each with different donor BMSCs, is shown. n.d. not detectable within the sensitivity of the assay, n.s. not significant within 35 cycles of amplification. Statistically significant differences are indicated (***p < 0.001)