Figure 7From: Long non-coding RNA ANRIL is upregulated in hepatocellular carcinoma and regulates cell apoptosis by epigenetic silencing of KLF2ANRIL negatively regulates expression of KLF2 by rescue assays. (A,B) Colony formation assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. (C) MTT assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. (D,E) The levels of KLF2 protein levels were determined by Western blot when HepG2 cells were transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2.*P < 0.05, **P < 0.01.Back to article page