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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Long non-coding RNA ANRIL is upregulated in hepatocellular carcinoma and regulates cell apoptosis by epigenetic silencing of KLF2

Figure 2

Effects of knockdown of ANRIL on HCC cell viability and apoptosis in vitro. (A) The ANRIL expression level was determined by qPCR when HepG2 and Hep3B cells were transfected with si-ANRIL. (B) MTT assays were used to determine the cell viability for si-ANRIL-transfected HepG2 and Hep3B cells. Values represented the mean ± s.d. from three independent experiments. (C) Colony formation assays were conducted to determine the proliferation of si-ANRIL-transfected HepG2 and Hep3B cells. (D) Flow cytometry assays were performed to analyze the cell cycle progression when HCC cells were transfected with si-ANRIL 24 h later. The bar chart represented the percentage of cells in G0/G1, S, or G2/M phase, as indicated. (E) Flow cytometry assays were performed to analyze the cell apoptosis when HCC cells were transfected with si-ANRIL 48 h later. *P < 0.05, **P < 0.01.

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