Figure 7
Azacytidine did not enhance hepatotoxicity of As2O3 in vitro. (A) Basal expression of AQP9 was much higher in MIHA compared with those of AML cells. (B) 5′Aza treatment did not increase AQP9 expression significantly in MIHA. (C) HNF1A promoter was hypomethylated in MIHA (upper panel), and 5′Aza treatment did not further increase HNF1A expression (lower panel). Sample data were normalized to the respective control. (D) Pre-treatment with 5′Aza did not significantly enhance the cytotoxicity of As2O3 in MIHA cells. Sample data were normalized to the respective control individually.