Figure 7

Oroxylin A-regulated glycolysis involved in the deacetylation of PTEN. (A) PC3M cells were co-transfected with wt-PTEN plasmids and siRNA SIRT3. Glucose uptake and lactate production were detected. (B) DU145 cells were co-transfected with PTEN siRNA and SIRT3 cDNA. Glucose uptake and lactate production were detected. (C, D) MCF-7 and HCT116 cells were transfected with siRNA targeting PTEN or with a non-targeting control siRNA, then incubated with 200 μM oroxylin A for 48 h. Lactate production (E) and glucose uptake (F) were detected. (E, F) MCF-7 and HCT116 cells were transfected with siRNA SIRT3, and then treated with 200 μM oroxylin A for 48 h, Lactate production (E) and glucose uptake (F) were detected. Bars, SD; *p < 0.05 or **p < 0.01.