Figure 6

WISH assays of cmyb in cebpa null mutants and siblings with or without dnmt1 mutation. (A) WISH assay of cmyb in embryos without cebpa and dnmt1 homozygote mutation. (B) WISH assay of cmyb in embryos only with dnmt1 homozygote mutation. Note that cmyb was markedly decreased. (C) WISH assay of cmyb in embryos only with cebpa homozygote mutation. (D) WISH assay of cmyb in embryos both with cebpa and dnmt1 homozygote mutation. Note that cmyb was comparable with embryos without dnmt1 homozygote mutation. (A’-D’) Magnified images of the boxed regions in A to D. Red arrows indicate cmyb-positive HSPCs in the CHT.