Figure 3

HDACIs triggered extrinsic apoptosis of T-lymphoma cells. A, Cell growth inhibition of Jurkat and H9 cells treated with different concentrations of HDACIs (VPA and SAHA) for 72 h was determined by MTT assay. B, Representative morphology of Jurkat and H9 cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h was revealed by Wright’s staining. C, Cell apoptosis was measured by Annexin-V/PI assay in Jurkat and H9 cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h. D, Caspase-8, cleaved caspase-8 and c-FLIP expression were detected by western blot. E, TRAIL and DR5 expression were detected by flow cytometry in Jurkat and H9 cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h. F, c-FLIP expression of Jurkat cells transfected with negative control (NC) or c-FLIP-overexpressing vector (Over) was confirmed by western blot (Left panel). Cell growth inhibition of these cells treated with different concentrations of HDACIs (VPA and SAHA) for 72 h was determined by MTT assay (Middle panel). Cell apoptosis of these cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h was measured by Annexin-V/PI assay (Right panel). *, P < 0.05 comparing with the negative control (NC).