Figure 4

BENC-511 activates apoptotic signaling in MM cells. (A) Myeloma cells (RPMI-8226, JJN3, OCI-MY5, U266, LP1) were treated with 4 μM BENC-511 or DMSO for 24 hours followed by the analysis of the expression of PARP, Caspase-3, and GAPDH. (B) OPM2, RPMI-8226 and LP1 cells were treated with increasing concentrations of BENC-511 for 24 hours. (C) OPM2 and RPMI-8226 were incubated with 4 μM of BENC-511 for the indicated time. After incubation, cells were harvested and total proteins were isolated. Cell lysates were applied for PARP and GAPDH analyses.