Figure 8

Selinexor (KPT-330) inhibits PCa cells-Induced osteoclast differentiation in the RAW264.7 cell model. (A) cytochemical evaluation of mouse TRAP in RAW264.7 cells treated with PC3 conditioned medium when these cells were cultured in the presence or not of 0.7 μM selinexor. This concentration was the IC20 value for RAW264.7 cell and was easily reached in vivo when 10 mg/Kg (q 2d × 3 weeks) KPT-330 was administered in the mice. Osteoclasts are large polinucleated TRAP + cells. (B) TRAP + polinucleated cells were counted and plotted in a graph considering a total count of 500 cells. (C) cells were collected and lysed to evaluate the enzymatic mTRAP activity. (D) IL-6, TGFb1 (E) and (F) RANL levels measured in osteotropic PC3 and PCb2 cells treated with 20 and 50 nM selinexor.