Skip to main content
Figure 4 | Journal of Hematology & Oncology

Figure 4

From: XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa)

Figure 4

Intratibial injection of PC3 cells. Treatments with KPT-251 and selinexor (KPT-330) started two day after cell inoculation. (A). Representative X-ray and bioluminescence pictures of untreated (vehicle) or KPT-251 and selinexor treated mice collected at 50th day after intratibial cell injection. (B) Tibiae injected with PC3M-pro4 luc cells from mice of controls (CTRL) and treated with KPT330 and visualized at low (100×) and high (400×) magnification. This panel presents in the upper side a hematoxylin/eosin staining showing a strong tumor cell colonization and very low/absent tumor cell colonization which is detected by immune-hystochemical evaluation of cytocheratin 18 (K18). In the CTRLs tumor cell form a compact structure with reduced bone stroma cell presence. Tumor cells colonize also the cortical bone after having supporting osteolysis. No PC3 tumor cell colonization associated with any macroscopic variations in the bone stroma cell population was evident after KPT330 treatments. Tr (bone trabecula), Bm (bone marrow), Tc (tumor cells). (C) graph showing the percentage of change in luciferase signal. (D) Number of radio logically evident intratibial bone lesions having scores ≤ 2 and ≥ 3 according to Yang et al. procedure[40]. (E) X ray scores. (F) osteoclast number evaluated in the bone metastatic lesions. Osteoclasts are visible as histochemical purple staining for TRAcP activity as a marker of osteoclasts. Data are expressed as number of osteoclasts/mm at the tumor–bone interface. Number of osteoclasts was proportional to the lysis score but no statistical significance was observed. NE = not evaluable. The NE cases were excluded by analyses. (G) mTRAP and (H) CTX-I levels in controls and upon 35 days of KPT-330 and KPT-251 treatments in intratibial injected mice with PC3 cells.

Back to article page