Figure 5
From: Identification of nuclear-enriched miRNAs during mouse granulopoiesis
Nuclear enrichment of miRNAs in primary mouse myeloid cells and hemopoietic cell lines. (A) RT-qPCR of known nuclear- and cytoplasmic-specific RNA in nuclear and cytoplasmic RNA fractions in mouse primary cells and cell lines. Lower panels show representative western blots for nuclear-specific Lmnb1 and cytoplasmic-specific Gapdh protein in the nuclear and cytoplasmic fractions. Both RT-qPCR and western blotting confirm the range of detection and enrichment of nuclear and cytoplasmic fractions (B) Nuclear-enriched miR-709, miR-706, miR-467a* and miR-690 in primary mouse LSK cells, promyelocytes, myelocytes and granulocytes. (C) Nuclear-enriched miRNAs in mouse hemopoietic cell lines, MPRO, EL4, MEL and A20. (* P < 0.05; ** P < 0.01, t-test). Prom; promyelocytes. Myel; myelocytes, Gran; granulocytes.