Figure 7

AGO2-related let-7a targets HIF-3α. The real-time PCR (A) and Western blot (B) detection of the HIF-3α expression levels in LP-1-si-AGO2 cells after transfection with a negative control and let-7a mimics. (C) The effect of let-7a on the luciferase activities of HIF-3α 3′-UTR or HIF-3α 3′-UTR with a mutated let-7a-binding site (mUTR). let-7a overexpression resulted in a significant decrease in the luciferase activity of the pmirGLO-reporter vector containing the HIF-3α 3′-UTR but not of the vector containing the corresponding mutant.