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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: Combination of the ABL kinase inhibitor imatinib with the Janus kinase 2 inhibitor TG101348 for targeting residual BCR-ABL-positive cells

Figure 3

Effects of imatinib and TG101348 on CD34-positive primary cells. (A) CD34-positive CML cells or healthy donor samples were cultured in the presence of HS-5 cells. Cells were then treated with varying concentrations of imatinib alone or in combination with TG101348 for 72 h. Viable cell numbers were calculated. Results are representative of three separate experiments. (B) CD34-positive primary cells were treated with the indicated concentrations of imatinib alone or in combination with TG101348 for 24 h. Total cell extracts were analyzed by immunoblotting using phospho-specific ABL, STAT5, Crk-L, and cleaved PARP Abs. β-actin was used as the loading control. Results are representative of at least three reproducible experiments. Blots were scanned and optical densities were determined using ImageJ software. (C) In vivo studies were performed as described in Methods. Tumors were treated with or without imatinib and TG101348 5 days per week, and growth was measured. *p < 0.05, compared with control mice.

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