Figure 1

xCT is highly expressed on KSHV-infected PEL cells. (A) Immunoblots were performed to identify xCT expression for 3 KSHV-infected PEL cell lines (BC-1, BCP-1 and BCBL-1). Expression of β-actin was assessed for loading controls. (B-C) PEL cells were transfected with negative control siRNA (n-siRNA) or siRNA targeting xCT for 48 h. Cells were then incubated with a monoclonal Ab recognizing an extracellular domain of xCT, followed by a secondary Ab conjugated to Alexa-647. PEL cell surface expression of xCT was then quantified by flow cytometry (B). The total xCT expression was measured by immunoblots (C).