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Figure 7 | Journal of Hematology & Oncology

Figure 7

From: CD44 standard and CD44v10 isoform expression on leukemia cells distinctly influences niche embedding of hematopoietic stem cells

Figure 7

Impact of EL4 and EL4-v10 on HSC marker and cell cycle-related protein expression. HSC (1×106) were cultured for 24 h on BSA or BM-Str coated 24-well plates; where indicated, cultures contained EL4 or EL4-v10 exosomes. HSC were collected and expression of (A) HSC markers and (B) cell cycle-related protein expression was evaluated by flow-cytometry; the mean ± SD (triplicates) percent of stained HSC and (C) representative examples are shown; significant differences (p <0.05) by the presence of BM-Str: *, significant differences (p <0.05) by EL4/EL4-v10 exosomes: s; the experiment was repeated 2-times. Expression of cell cycle promoting genes is reduced in HSC cultured on BM-Str, but EL4 exosomes negate the effect of BM-Str on cyclinD1 and c-myc expression.

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