Figure 3

Impact of EL4 and EL4-v10 exosomes on stroma cells. (A,B) Flow-cytometry of EL4 and EL4-v10 cells and exosomes; mean percent stained cells ± SD (triplicates) and representative example; significant differences between cells versus exosomes: *. (C) Flow cytometry of BM-Str and spleen-Str and (D,E) of BM-Str treated for 24 h with EL4 and EL4-v10 exosomes; mean ± SD percent stained Str (triplicates) and representative examples; significant changes (p <0.05) by coculture with EL4 or EL4-v10 exo: s. Experiments A-E were repeated 3-times. BM- and spleen-Str are highly CD44⁺ and express stroma cell markers, there was no contamination by hematopoietic cells. EL4 and EL4-v10 exosomes promoted upregulation of TNFRI and of the chemokines SDF1, IP10, Rantes and OPN, only expression of the latter becoming more strongly induced by EL4-v10 than EL4.