Figure 6
As 2 O 3 down-regulated GLI1 expression via the Hedgehog signaling pathway. (A), As2O3 decreased GLI1 protein expression in CD133+ Huh7-wt and CD133+ Hep3B-wt cells, as indicated by Western blotting analysis. (B), Short hairpin RNA knockdown of GLI1 expression decreased CD133 expression in CD133+ Huh7-wt and CD133+ Hep3B-wt cells; similarly, up-regulation of GLI1 expression increased CD133 expression (wt = CD133+ Huh7-wt and CD133+ Hep3B-wt; control = Huh7-LV-shNon, Hep3B-LV-shNon, and Huh7-LV-oeNon, Hep3B-LV-oeNon; sh = Huh7-LV-shGLI1 and Hep3B-LV-shGLI1; oe = Huh7-LV-oeGLI1 and Hep3B-LV-oeGLI1). (C), Huh7-LV-shGLI1 and Hep3B-LV-shGLI1 cells were treated with 4 μM As2O3 for 48 hours, the expression of CD133 did not decrease significantly.