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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: ATP directed agent, 8-chloro-adenosine, induces AMP activated protein kinase activity, leading to autophagic cell death in breast cancer cells

Figure 4

8-Cl-Ado inhibits the activity of the mTOR pathway. Immunoblot analysis of 8-Cl-Ado-induced (A) phosphoylation of raptor on the AMPK phosphorylation site, Ser792, as compared with total raptor and (B) specific inhibition of an mTOR phosphorylation site on 4E-BP1 Ser65 along with compression of slower migrating hyperphosphorylated 4E-BP1 bands in MCF-7 and BT-474 cells treated with 10 μM of 8-Cl-Ado for the indicated times. The normalized ratios of p-raptor Ser792 to GAPDH and of p-4E-BP1 Ser65 to total relative to the untreated control is indicated beneath their respective lane. GAPDH was used as loading controls for all blots.

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